"Typical" chemical compositions have been determined on media ingredients. The typical analysis is used to select products for research or production needs when specific nutritional characteristics are required. The specifications for the typical analysis include :

• Physical characteristics,
• Nitrogen content,
• Amino acids,
• Inorganics,
• Vitamins, and
• Biological testing.            

All values are presented as weight/weight; % = g/100 g.


Glossary

Ash

The higher the ash content, the lower the clarity of the prepared ingredient. The ash content includes sodium chloride, sulfate, phosphates, silicates and metal oxides. Acid-insoluble ash is typically from silicates found in animal fodder.


Moisture

Lower moisture levels (<.5%) are preferred. Higher moisture levels in dehydrated ingredients may reduce stability. In the presence of high moisture and high ambient temperatures, chemical interactions will cause darkening of the product and falling pH. These characteristics indicate product deterioration.

Nitrogen

Total Nitrogen : Total nitrogen is usually measured by the kjeldhal digestion or titration method. Not all organic nitrogen is nutritive. Percent (%) nitrogen x 6.25 ~ % proteins, peptides or amino acids present.

Amino Nitrogen : The amino nitrogen value shows the extent of protein hydrolysis by measuring the increase in free amino groups. This is a nutritionally meaningful value.

pH

Changes in pH from specified values, either after storage or processing, indicate deterioration. These changes are uaually accompanied by darkening of the end product. Hydrolysates vary in their pH resistance according to their inherent buffering (phosphate) capacity.

Phosphates

High-phosphate ingredients may be unsuitable for pH indicator media due to the inherent buffering of phosphates. However, phosphates do aid in gas production, which can be enhanced by deliberate addition of sodium phosphate.

Sodium Chloride

The NaCl content may reflect significant pH adjustments during processing e.g., acid hydrolysates. (See Ash).

Trace Metals

Trace metals can directly antagonize antimicrobial activity in vitro or impact toxin production, chelating agents may be added to culture media to sequester trace metals and clarify the media.

Product Doucmentation

Certificate of Analysis : For any given product and its batch number, a COA can be requested for.

Material Safety Data Sheet : To ensure proper handling and storage of the product, MSDS is also abailable for each Microgen media product.

Specification Sheet : Product specifications may vary from those printed on the specification manual. To obtain the latest updated information for any product that you wish to purchase, kindly request a specification sheet or simply log on to our website and download copy.